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Clinical Studies

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PhytoCellTec™ Malus Domestica
Plant stem cells for skin stem cell protection
 

A Revolutionary Technology to Protect Skin Stem Cells PhytoCellTec™ Malus Domestica is a liposomal pre paration based on the stem cells of a rare Swiss apple.

Uttwiler Spãtlauber is an endangered apple variety that was well-known for its excellent storability and thus its longevity potential.

Mibelle Biochemistry has developed a novel technology enabling the cultivation of rare and endangered species like Uttwiler Spãtlauber. Thanks to this technology called PhytoCellTec™, plant stem cells can be obtained and incorporated into cosmetic products to ensure the longevity of skin cells.

Studies showed the double activity of PhytoCellTec™ Malus Domestica:

 

  • it helps skin stem cells to maintain their characteristics and their capacity to build new tissues
  • it delays aging and has an anti-wrinkle effect.

PhytoCellTec™ Malus Domestica is the first plant stem cell active ingredient on the market whose effect was evaluated on human skin stem cells. This unique and revolutionary ingredient is able to protect the most precious skin cells, the skin stem cells, against premature aging.

 

Claim Ideas for PhytoCellTec™ Malus Domestica

  • Protects longevity of skin stem cells
  • Delays senescence of essential cells
  • Increases the vitality of skin stem cells
  • Combats chronological aging

 

 

 

 

Claim Ideas for PhytoCellTec™ Malus Domestica

  • Protects longevity of skin stem cells
  • Delays senescence of essential cells
  • Increases the vitality of skin stem cells
  • Combats chronological aging

Applications

  • Advanced "stem cell cosmetic" formulas
  • Real rejuvenation for face and body care
  • Innovative skin care formulations

Formulating with PhytoCellTec™ Malus Domestica

  • Recommended use level: 2 - 5%
  • Incorporation: For cold processes, mix PhytoCellTec™Malus Domestica into the aqueous phase. In cold / hotprocesses, add during the cooling phase below 40° C.s
  • Thermostability: Temperatures of up to 60° C for a short time do not affect the stability of PhytoCellTec™ Malus Domestica.

INCI / CTFA-Declaration

Malus Domestica Fruit Cell Culture Extract (and) Xanthan Gum (and) Glycerin (and) Lecithin (and) Phenoxyethanol (and) Aqua/ Water

Additional Information

  • PhytoCellTec™ Malus Domestica contains 9% of Malus Domestica stem cell extract
  • Available in a phenoxyethanol-free version
  • Available in powder form
 
PhytoCellTec™ Malus Domestica
Swiss apple stem cells to protect skin stem cells

A Rare Apple with Incredible Properties

Uttwiler Spãtlauber is a variety of a Swiss apple that derives from a seedling planted in the middle of the 18th century. It was very famous for its excellent storability without shriveling. Today apple cultivars are selected to maximize crop yield and sweet flavor. Thus Uttwiler Spãtlauber with its acid taste is now disappearing.

Uttwiler Spãtlauber apples are rich in phytonutrients, proteins and long-living cells. They must have especially long-living tissue stem cells. Their particular composition leads to incredible storability and longevity properties.

Stem Cells and Longevity

Longevity is related to specific cells called stem cells which have a unique growth characteristic. These unspecialized (undifferentiated) cells can make identical copies of themselves as well as differentiate to become specialized cells.

 Stem Cells in the Human Skin

In the human skin, two types of adult stem cells havebeen identified:

  • epithelial skin stem cells which are located in the basal layer of the epidermis
  • hair bulge stem cells located in the hair follicle.

Fast regenerating tissues such as the epidermis are particularly dependent on their stem cells because only stem cells retain the capacity to divide and thus to provide new tissues.

Epidermal stem cells have 2 main functions:

  • replenish and maintain the balance of cells within the skin
  • regenerate damaged tissues.

Two basic types of stem cells are present in the humanbody:

  • embryonic stem cells found in blastocysts can grow and differentiate into one of the more than 220 different cell types which make up the human body.
  • adult stem cells located in some adult tissues can only differentiate into their own or related cell types. These cells act as a repair system for the body but also maintain the normal turnover of regenerative organs such as blood, skin or intestinal tissues.

 

Skin Stem Cells and Aging

Two basic types of stem cells are present in the humanbody:

  • Skin stem cells have a limited life expectancy.
  • Intrinsic or extrinsic stress factors can affect their functionality.
  • With age, skin stem cells are less active and lower in number.

Plant Stem Cells to Protect Skin Stem Cells

All stem cells, independently of their origin (plant, animal or human) contain specific epigenetic factors whose function is to maintain the self-renewal capacity of stem cells. Therefore the Malus Domestica stem cells are used to help to preserve the vitality of human skin stem cells.

 

 

 

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PhytoCellTec™ Malus Domestica
Advanced biotechnology to cultivate cells from rare plants

PhytoCellTec™ by Mibelle Biochemistry

Mibelle Biochemistry has developed a novel technology called PhytoCellTec™, enabling the large scale cultivation of cells from rare and endangered plant species.

This technology is based on the unique totipotency of plant cells that is to say:

  • the capacity of every plant cell to regenerate new organs or even the whole plant
  • the capacity of every plant cell to dedifferentiate and become a stem cell.

Our PhytoCellTec™ technology relies on the wound healing mechanism of a plant: part of a plant is wounded to induce the formation of callus cells. This healing tissue consists of dedifferentiated cells which are stem cells. Callus cells are harvested and cultivated in a suspension. A novel bioreactor system enables the large scale production.

 

 

 

To obtain the PhytoCellTec™ Malus Domestica cosmetic ingredient, these stem cells are harvested and homogenized at 1200 bar together with phospholipids to encapsulate and stabilize oil- and water-soluble components into liposomes.

PhytoCellTec™ Malus Domestica is thus rich in epigenetic factors and metabolites which assure the longevity of cells and protect stem cells.

Advantages of PhytoCellTec™ Technology

This innovative technology developed by Mibelle Biochemistry offers the following advantages:

  • possibility to cultivate cells of rare and endangered plants based on small amounts of origin plant material
  • availability of plant material independent of the season and market demand
  • plant material completely free of environmental pollutants and pesticides
  • constant concentrations of metabolites in the stem cells

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PhytoCellTec™ Malus Domestica
Study results

Preparation of Epidermal Stem Cells

A novel Progenitor Cell Targeting technology was used toprepare
human epidermal stem cells.

This technology consists of culturing a skin sample in a medium specifically
designed to mimic the microenvironment of the stem cell in the epidermis.

This technology is based on the unique totipotency of plant cells that is to
say:

This special, fully defined cell culture medium leads to an enrichment of so
called keratinocyte progenitor cells that can be considered as stem cells.
This enrichment was quantitatively controlled through FACS
(Fluorescenceactivated cell sorting) of cells which were labelled with CD34
and a6 integrin, two well-known markers of epidermal stem cells. Compared
to freshly isolated cells, the cell population of passage 3 was characterized
by a 10- fold increase of CD34 /a6 integrin double labelled cells.

The Progenitor Cell Targeting technology provides a cell culture model which
specifically enables the evaluation of the effects of compounds on epidermal
stem cells.

The Capacity to Form a Colony is a Characteristic of Stem Cells

Stem cells have the characteristic to form colonies in vitro.

A colony consists of:

  •  the progenitor cell
  •  transient amplifying cells (cells in an intermediate state)
  •  differentiated cells that have lost the capacity to divide.
The number of colonies formed is a value of the vital progenitor /stem cells
and is called colony forming efficiency (CFE). To measure CFE, cells are
seeded at a low density.